Decondensation of Bull and Human Sperm Nuclei by Dithiothreitol and/or Heparin
نویسندگان
چکیده
منابع مشابه
Improved bovine ICSI outcomes by sperm selected after combined heparin-glutathione treatment.
Despite widespread application of intracytoplasmic sperm injection (ICSI) in human-assisted reproductive techniques (ART), the efficiency of this method is still far from satisfactory in livestock, particularly in the bovine species with its unique sperm condensation. On the basis of the natural chemical structure of chromatin in condensed sperm, we developed a novel combined heparin-reduced gl...
متن کاملFurther evidence on the role of heparan sulfate as protamine acceptor during the decondensation of human spermatozoa.
BACKGROUND Human spermatozoa decondense in vitro upon exposure to heparin and glutathione. Glutathione is also the disulfide bond reducer in vivo, and heparan sulfate, a functional analogue of heparin, has been proposed as the protamine acceptor. The aim of this study was to evaluate the decondensing ability of chemically modified heparins and different glycosaminoglycans (GAGs) on isolated spe...
متن کاملIn vivo and in vitro decondensation of human sperm and assisted reproduction technologies.
For a successful fertilization and pronucleus formation to occur, not only a properly condensed sperm nucleus, but also decondensation ability in the oocyte is important. The sperm abnormalities causing failure of sperm decondensation in the oocyte are unrecognizable by conventional semen analysis and different methods are used. The chromatin decondensation ability of the human spermatozoa in v...
متن کاملBull Fertility and Its Relation with Density Gradient Selected Sperm
Background sperm selection is generally used to describe methods for separation of spermatozoa for in-vitro-assisted reproduction. Few studies reported relation in vivo fertility and semen parameters after selection of fertile spermatozoa; hence, our research attempt to assess different semen parameters after post-thaw (PT) or after selecting sperm sample (SSp), using density gradient separatio...
متن کاملP-226: American Ginseng Effects on Damage Induced by Cyclophosphamide in The Nuclei of Rat Sperm
Background: Oxidative stress occurs when the oxidative homeostasis is damage. Excessive ROS are generated and this reaction has been proposed to produce marked damage to the structure and function of sperm. Materials and Methods: This study was designed to investigate the protective effects of of American Ginseng against cyclophosphamide - induced damage in the rat sperm. For this experiment, t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 1996
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.42.7